George Crabtree

Glucose transport in crabtree-positive and crabtree-negative yeasts.

The kinetic parameters of glucose transport in four Crabtree-positive and four Crabtree-negative yeasts were determined. The organisms were grown in aerobic glucose-limited chemostats at a dilution rate of 0.1 h-1. The results show a clear correlation between the presence of high-affinity glucose transport systems and the absence of aerobic fermentation upon addition of excess glucose to steady...

The Crabtree effect: a review.

Relief of the Crabtree Effect by Arsenate1

Arsenate, an uncoupler of oxidative phosphorylation, relieved the Crabtree effect in Ehrlich-Lett réhyperdiploid ascites tumor cells. Respiration was measured with an oxygen electrode. High arsenale concentrations (30 to 60 ITIM)were needed to produce a 2-fold increase in the depressed respiratory rate. Arsenate concentrations below 10 HTMhad little or no effect. The relief by arsenale was rev...

An evolutionary perspective on the Crabtree effect

The capability to ferment sugars into ethanol is a key metabolic trait of yeasts. Crabtree-positive yeasts use fermentation even in the presence of oxygen, where they could, in principle, rely on the respiration pathway. This is surprising because fermentation has a much lower ATP yield than respiration (2 ATP vs. approximately 18 ATP per glucose). While genetic events in the evolution of the C...

George

not much worried whether they be right or wrong. His desire to be the center of attraction may lead him to do things considered irrational when viewed from other angles. If he is in the upper 10 per cent of his class at school, it might be well to advance him to the next grade, provided he is sufficiently interested to show that he is capable of doing the work. If convenient, the teacher should...